Coupling SAR C-band and optical data for soil moisture and leaf area index retrieval over irrigated grasslands

International audienceThe objective of this study was to develop an approach for estimating soil moisture and vegetation parameters in irrigated grasslands by coupling C-band polarimetric Synthetic Aperture Radar (SAR) and optical data. A huge dataset of satellite images acquired from RADARSAT-2 and LANDSAT-7/8, and in situ measurements were used to assess the relevance of several inversion configurations. A neural network (NN) inversion technique was used. The approach for this study was to use RADARSAT-2 and LANDSAT-7/8 images to investigate the potential for the combined use of new data from the new SAR sensor SENTINEL-1 and the new optical sensors LANDSAT-8 and SENTINEL-2. First, the impact of SAR polarization (mono-, dual- and full-polarizations configurations) and the Normalized Difference Vegetation Index (NDVI) calculated from optical data for the estimation error of soil moisture and vegetation parameters was studied. Next, the effect of some polarimetric parameters (Shannon entropy and Pauli components) on the inversion technique was also analyzed. Finally, configurations using in situ measurements of the fraction of absorbed photosynthetically active radiation (FAPAR) and the fraction of green vegetation cover (FCover) were also tested.The results showed that HH polarization is the SAR polarization most relevant to soil moisture estimates. An RMSE for soil moisture estimates of approximately 6 vol.% was obtained even for dense grassland cover. The use of in situ FAPAR and FCover only improved the estimate of the leaf area index (LAI) with an RMSE of approximately 0.37 m²/m². The use of polarimetric parameters did not improve the estimate of soil moisture and vegetation parameters. Good results were obtained for the biomass (BIO) and vegetation water content (VWC) estimates for BIO and VWC values lower than 2 and 1.5 kg/m², respectively (RMSE is of 0.38 kg/m² for BIO and 0.32 kg/m² for VWC). In addition, a high under-estimate was observed for BIO and VWC higher than 2 and 1.5 kg/m², respectively (a bias of -0.65 kg/m² on BIO estimates and -0.49 kg/m² on VWC estimates). Finally, the estimation of vegetation height (VEH) was carried out with an RMSE of 13.45 cm

Effect of gamma radiation on ultraviolet, visible and infrared studies of NiO, Cr2O3 and Fe2O3-doped alkali borate glasses

AbstractBorate glasses with basic composition xR2O.(100−x)B2O3 where R=Na, K, were colored by doping with one of transition metal ions Ni, Cr, Fe or doping with mixing of them. The effect of glass composition and gamma ray irradiation on the optical and infrared absorption spectra was studied and interpreted in terms of structural concepts. A resolution of the observed absorption spectra showed the existence of each transition metal ion in the possible coordination state according to the composition of the glass. The IR measurements reveal characteristic absorption bands due to various groups of triangular and tetrahedral borate network. The introduction of trace amounts of mixed dopants Cr2O3, Fe2O3 and NiO has no major effect on the structure of the studied glasses. The induced absorption spectra exhibit the characteristic absorption bands caused by the intrinsic base borate glass and the respective transition metal ions. The response of the doped glasses to gamma ray irradiation is assumed to be related to the formation and annihilation of the induced color centers

A protocol for primary isolation and culture of adipose-derived stem cells and their phenotypic profile

Background: Adipose tissue (AT) is a rich source of mesenchymal stem cells (MSCs), however, there is no standardized protocol for stem cell isolation and culture. This leads to inconsistency of the results and limits the comparison of the data from different laboratories. Our aim was to provide an applied protocol for ASCS isolation and expansion, study the cell behavior and define their cellular surface markers. ASCs were cultured from both resected adipose tissue (RAT) obtained following abdominoplasty or breast reduction and lipoaspirates (LPA) following laser-free liposuction. Method: the protocol entailed coculturing of stromal vascular fraction (SVF) with RAT as raw pieces using DMEM medium with varying glucose concentration. The coculture protocol aimed to mimic the normal physiological conditions required for cell growth. ASCs were immunophenotyped to define their MSCs surface markers by flowcytometry. Results: ASCs were isolated from coculturing RAT with SVF with fibroblast-like adherent cells morphology. The ASCs yield isolated from LPA was significantly greater than from RAT on day 14 and 28 (p = 0.002, <0.001, respectively). Significant increase in ASCs proliferation rate was detected when ASCs were cultured under high glucose (4.5 g/L) compared to low glucose (1 g/ L) condition on day 7 and 14 (p = 0.04, 0.015, respectively). ASCs isolated from both protocols were positive for CD34, CD49d, CD73, CD90 and CD105 and negative for CD3, CD14, CD19, CD45 and HLA-DR. Conclusion: We concluded that the cells harvested by our protocol were ASCs. Hence, our method can be an efficient isolation tool to obtain primary ASCs under culture conditions mimicking normal physiological status. This will help in providing ASCs which can be similar to cells in human tissue for further study

Coupling potential of ICESat/GLAS and SRTM for the discrimination of forest landscape types in French Guiana

The Shuttle Radar Topography Mission (SRTM) has produced the most accurate nearly global elevation dataset to date. Over vegetated areas, the measured SRTM elevations are the result of a complex interaction between radar waves and tree crowns. In this study, waveforms acquired by the Geoscience Laser Altimeter System (GLAS) were combined with SRTM elevations to discriminate the five forest landscape types (LTs) in French Guiana. Two differences were calculated: (1) penetration depth, defined as the GLAS highest elevations minus the SRTM elevations, and (2) the GLAS centroid elevations minus the SRTM elevations. The results show that these differences were similar for the five LTs, and they increased as a function of the GLAS canopy height and of the SRTM roughness index. Next, a Random Forest (RF) classifier was used to analyze the coupling potential of GLAS and SRTM in the discrimination of forest landscape types in French Guiana. The parameters used in the RF classification were the GLAS canopy height, the SRTM roughness index, the difference between the GLAS highest elevations and the SRTM elevations and the difference between the GLAS centroid elevations and the SRTM elevations. Discrimination of the five forest landscape types in French Guiana was possible, with an overall classification accuracy of 81.3% and a kappa coefficient of 0.75. All forest LTs were well classified with an accuracy varying from 78.4% to 97.5%. Finally, differences of near coincident GLAS waveforms, one from the wet season and one from the dry season, were analyzed. The results showed that the open forest LT (LT12), in some locations, contains trees that lose leaves during the dry season. These trees allow LT12 to be easily discriminated from the other LTs that retain their leaves using the following three criteria: (1) difference between the GLAS centroid elevations and the SRTM elevations, (2) ratio of top energy in the wet season to top energy in the dry season, or (3) ratio of ground energy in the wet season to ground energy in the dry season

Capability of GLAS/ICESat data to estimate forest canopy height and volume in mountainous forests of Iran

International audienceThe importance of measuring biophysical properties of forest for ecosystem health monitoring and forest management encourages researchers to find precise, yet low cost methods especially in mountainous and large area. In the present study Geoscience Laser Altimeter System (GLAS) on board ICESat was used to estimate three biophysical characteristics of forests located in north of Iran: 1) maximum canopy height (Hmax), 2) Lorey's height (HLorey), and 3) Forest volume (V). A large number of Multiple Linear Regressions (MLR) and also Random Forest (RF) regressions were developed using different set of variables: waveform metrics, Principal Components (PCs) produced from Principal Component Analysis (PCA) and Wavelet Coefficients (WCs) generated from wavelet transformation. To validate and compare different models, statistical criteria were calculated based on a five-fold cross validation. The best model concerning the maximum canopy height was an MLR with an RMSE of 5.0 m which combined two metrics extracted from waveforms (waveform extent "Wext" and height at 50% of waveform energy "H50"), and one from the Digital Elevation Model (Terrain Index: TI). The mean absolute error (MAPE) of maximum canopy height estimates is about 16.4%. For Lorey's height, a simple MLR model including two metrics (Wext and TI) represents the highest performance (RMSE=5.1 m, MAPE=24.0%). Totally, MLR models showed better performance rather than RF models, and accuracy of height estimations using waveform metrics was greater than those based on PCs or WCs. Concerning forest volume, employing regression models to estimate volume directly from GLAS data led to a better result (RMSE=128.8 m3/ha) rather than volume-HLorey relationship (RMSE=167.8 m3/ha)

Regional scale rain-forest height mapping using regression-kriging of spaceborne and airborne lidar data : application on French Guiana

LiDAR data has been successfully used to estimate forest parameters such as canopy heights and biomass. Major limitation of LiDAR systems (airborne and spaceborne) arises from their limited spatial coverage. In this study, we present a technique for canopy height mapping using airborne and spaceborne LiDAR data (from the Geoscience Laser Altimeter System (GLAS)). First, canopy heights extracted from both airborne and spaceborne LiDAR were extrapolated from available environmental data. The estimated canopy height maps using Random Forest (RF) regression from airborne or GLAS calibration datasets showed similar precisions (~6 m). To improve the precision of canopy height estimates, regression-kriging was used. Results indicated an improvement in terms of root mean square error (RMSE, from 6.5 to 4.2 m) using the GLAS dataset, and from 5.8 to 1.8 m using the airborne LiDAR dataset. Finally, in order to investigate the impact of the spatial sampling of future LiDAR missions on canopy height estimates precision, six subsets were derived from the initial airborne LiDAR dataset. Results indicated that using the regression-kriging approach a precision of 1.8 m on the canopy height map was achievable with a flight line spacing of 5 km. This precision decreased to 4.8 m for flight line spacing of 50 km

Regional scale rain-forest height mapping using regression-kriging of spaceborneand airborne lidar data: application on French Guiana

IGARSS 2015, Milan, ITA, 26-/07/2015 - 31/07/2015International audienceLiDAR remote sensing has been shown to be a good technique for the estimation of forest parameters such as canopy heights and aboveground biomass. Whilst airborne LiDAR data are in general very dense but only available over small areas due to the cost of their acquisition, spaceborne LiDAR data acquired from the Geoscience Laser Altimeter System (GLAS) have a coarser acquisition density associated with a global cover. It is therefore valuable to analyze the integration relevance of canopy heights estimated from LiDAR sensors with ancillary data such as geological, meteorological, and phenological variables in order to propose a forest canopy height map with good precision and high spatial resolution.In this study, canopy heights extracted from both airborne and spaceborne LiDAR, were first extrapolated from available environmental data. The estimated canopy height maps using random forest (RF) regression from the airborne or GLAS calibration datasets showed similar precisions (RMSE better than 6.5 m). In order to improve the precision of the canopy height estimates regression-kriging (kriging of RF regression residuals) was used. Results indicated an improvement in the RMSE (decrease from 6.5 to 4.2 m) for the regression-kriging maps from the GLAS dataset, and from 5.8 to 1.8 m for the regression-kriging map from the airborne LiDAR dataset